A Modified Alkaline Comet Assay for Measuring DNA Repair Capacity in Human Populations
نویسندگان
چکیده
منابع مشابه
Studies on electron beam induced DNA damage and repair kinetics in lymphocytes by alkaline comet assay
Background: Exposure to ionizing radiation is known to induce oxidative stress followed by damage to critical biomolecules like lipids, proteins and DNA through radiolysis of cellular water. Since radiation has been widely used as an important tool in therapy of cancer, the detailed investigation regarding the DNA damage and repair kinetics would help to predict the radiation sensitivity of cel...
متن کاملAssessment of human DNA repair (NER) capacity with DNA repair rate (DRR) by comet assay.
OBJECTIVE Alkaline comet assay was used to evaluate DNA repair (nucleotide excision repair, NER) capacity of human fresh lymphocytes from 12 young healthy non-smokers (6 males and 6 females). METHODS Lymphocytes were exposed to UV-C (254 nm) at the dose rate of 1.5 J/m2/sec. Novobiocin (NOV) and aphidicolin (APC), DNA repair inhibitors, were utilized to imitate the deficiency of DNA repair ca...
متن کاملA modified alkaline Comet assay for in vivo detection of oxidative DNA damage in Drosophila melanogaster.
Modifications to the alkaline Comet assay by using lesion-specific endonucleases, such as formamidopyrimidine-DNA glycosylase (FPG) and endonuclease III (ENDOIII, also known as Nth), can detect DNA bases with oxidative damage. This modified assay can be used to assess the genotoxic/carcinogenic potential of environmental chemicals. The goal of this study was to validate the ability of this modi...
متن کاملstudies on electron beam induced dna damage and repair kinetics in lymphocytes by alkaline comet assay
background: exposure to ionizing radiation is known to induce oxidative stress followed by damage to critical biomolecules like lipids, proteins and dna through radiolysis of cellular water. since radiation has been widely used as an important tool in therapy of cancer, the detailed investigation regarding the dna damage and repair kinetics would help to predict the radiation sensitivity of cel...
متن کاملSingle Cell Analysis of Human RAD18-Dependent DNA Post-Replication Repair by Alkaline Bromodeoxyuridine Comet Assay
Damage to DNA can block replication progression resulting in gaps in the newly synthesized DNA. Cells utilize a number of post-replication repair (PRR) mechanisms such as the RAD18 controlled translesion synthesis or template switching to overcome the discontinuities formed opposite the DNA lesions and to complete DNA replication. Gaining more insights into the role of PRR genes promotes better...
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ژورنال
عنوان ژورنال: Radiation Research
سال: 2008
ISSN: 0033-7587,1938-5404
DOI: 10.1667/rr1101.1